Tailoring the volatility and stability of oligopeptides

Author(s)
J. Schatti, U. Sezer, S. Pedalino, J. P. Cotter, M. Arndt, M. Mayor, V. Köhler
Abstract

Amino acids are essential building blocks of life, and fluorinated derivatives have gained interest in chemistry and medicine. Modern mass spectrometry has enabled the study of oligo- and polypeptides as isolated entities in the gas phase, but predominantly as singly or even multiply charged species. While laser desorption of neutral peptides into adiabatically expanding supersonic noble gas jets is possible, UV–VIS spectroscopy, electric or magnetic deflectometry as well as quantum interferometry would profit from the possibility to prepare thermally slow molecular beams. This has typically been precluded by the fragility of the peptide bond and the fact that a peptide would rather ‘fry’, i.e. denature and fragment than ‘fly’. Here, we explore how tailored perfluoroalkyl functionalization can reduce the intermolecular binding and thus increase the volatility of peptides and compare it to previously explored methylation, acylation and amidation of peptides. We show that this strategy is essential and enables the formation of thermal beams of intact neutral tripeptides, whereas only fragments were observed for an extensively fluoroalkyl-decorated nonapeptide.

Organisation(s)
Quantum Optics, Quantum Nanophysics and Quantum Information
External organisation(s)
Universität Basel, Karlsruher Institut für Technologie
Journal
Journal of Mass Spectrometry
Volume
52
Pages
550-556
No. of pages
7
ISSN
1076-5174
DOI
https://doi.org/10.1002/jms.3959
Publication date
08-2017
Peer reviewed
Yes
Austrian Fields of Science 2012
103006 Chemical physics
Keywords
ASJC Scopus subject areas
Spectroscopy
Portal url
https://ucrisportal.univie.ac.at/en/publications/tailoring-the-volatility-and-stability-of-oligopeptides(d4976fa2-321a-41c7-815c-6d4fb43586ab).html