Laser-Induced Acoustic Desorption of Natural and Functionalized Biochromophores
- Author(s)
- Ugur Sezer, Lisa Woerner, Johannes Horak, Lukas Felix, Jens Tüxen, Christoph Goetz, Alipasha Vaziri, Marcel Mayor, Markus Arndt
- Abstract
Laser-induced acoustic desorption (LIAD) has recently been established as a tool for analytical chemistry. It is capable of launching intact, neutral, or low charged molecules into a high vacuum environment. This makes it ideally suited to mass spectrometry. LIAD can be used with fragile biomolecules and very massive compounds alike. Here, we apply LIAD time-of-flight mass spectrometry (TOF-MS) to the natural biochromophores chlorophyll, hemin, bilirubin, and biliverdin and to high mass fluoroalkyl-functionalized porphyrins. We characterize the variation in the molecular fragmentation patterns as a function of the desorption and the VUV postionization laser intensity. We find that LIAD can produce molecular beams an order of magnitude slower than matrix-assisted laser desorption (MALD), although this depends on the substrate material. Using titanium foils we observe a most probable velocity of 20 m/s for functionalized molecules with a mass m = 10 000 Da.
- Organisation(s)
- Quantum Optics, Quantum Nanophysics and Quantum Information, Max Perutz Labs
- External organisation(s)
- Universität Basel, Karlsruher Institut für Technologie
- Journal
- Analytical Chemistry
- Volume
- 87
- Pages
- 5614-5619
- No. of pages
- 6
- ISSN
- 0003-2700
- DOI
- https://doi.org/10.1021/acs.analchem.5b00601
- Publication date
- 06-2015
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 103016 Laser physics, 104002 Analytical chemistry
- Keywords
- ASJC Scopus subject areas
- Analytical Chemistry
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/e98ab944-df1e-4bb0-b875-58eb13068b1f